7YU1
Structure of 6-aminohexanoate-oligomer hydrolase NylC precursor, D122G/H130Y/T267C mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12-12 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 69.879, 144.004, 129.284 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.269 - 1.130 |
| Rwork | 0.129 |
| R-free | 0.15530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3axg |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.807 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.150 |
| High resolution limit [Å] | 1.130 | 1.130 |
| Rmerge | 0.055 | 0.647 |
| Rmeas | 0.059 | 0.703 |
| Number of reflections | 240917 | 11845 |
| <I/σ(I)> | 33.9 | 3.22 |
| Completeness [%] | 99.5 | 98.6 |
| Redundancy | 7 | 6.5 |
| CC(1/2) | 0.999 | 0.904 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 283 | 1.4 M ammonium sulfate, 0.2 M sodium chloride, 0.1 M HEPES pH 7.5 |
