7YU0
Structure of 6-aminohexanoate-oligomer hydrolase NylC precursor, H130Y/N266A/T267A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-05-22 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 70.242, 144.345, 129.178 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.006 - 1.350 |
| Rwork | 0.111 |
| R-free | 0.13940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3axg |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.492 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.370 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.074 | 0.499 |
| Rmeas | 0.077 | 0.526 |
| Number of reflections | 140625 | 5859 |
| <I/σ(I)> | 30.4 | 3.35 |
| Completeness [%] | 98.0 | 82 |
| Redundancy | 12.5 | 8.7 |
| CC(1/2) | 0.999 | 0.940 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 283 | 1.4 M ammonium sulfate, 0.2 M sodium chloride, 0.1 M HEPES pH 7.5 |
