7YTU
Crystal structure of vaccinia virus G3/L5 sub-complex (SeMet-labeled, P31 space group)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-10-05 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97892 |
Spacegroup name | P 31 |
Unit cell lengths | 54.039, 54.039, 42.742 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 31.560 - 1.504 |
R-factor | 0.188 |
Rwork | 0.187 |
R-free | 0.20310 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 0.955 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXDE |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.550 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.109 | 0.611 |
Number of reflections | 22077 | 2153 |
<I/σ(I)> | 19.46 | 2.43 |
Completeness [%] | 99.6 | 96.7 |
Redundancy | 7.5 | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 0.1 M Sodium formate (pH 7.0), 12% w/v Polyethylene glycol 3350 |