7YTH
Structure of OCPx1 from Nostoc flagelliforme CCNUN1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-01-15 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97935 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 79.780, 122.950, 141.460 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 67.010 - 1.930 |
| R-factor | 0.2017 |
| Rwork | 0.199 |
| R-free | 0.24780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4xb5 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.609 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 79.780 | 1.980 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Rmerge | 0.067 | 0.671 |
| Number of reflections | 103819 | 7107 |
| <I/σ(I)> | 15.5 | 3.1 |
| Completeness [%] | 98.7 | 92.8 |
| Redundancy | 6.6 | 5.6 |
| CC(1/2) | 0.998 | 0.854 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2 M Ammonium acetate, 0.1 M HEPES pH 7.5, 25% w/v Polyethylene glycol 3,350 |
