7YT0
Crystal Structure of UDP-glucose 4-epimerase (Rv3634c) in complex with UDP-galactose from Mycobacterium tuberculosis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E+ SUPERBRIGHT |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2020-08-19 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 55.796, 81.514, 146.867 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.090 - 1.760 |
R-factor | 0.1822 |
Rwork | 0.181 |
R-free | 0.21110 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7ys8 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.934 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.790 |
High resolution limit [Å] | 1.760 | 1.760 |
Rmerge | 0.061 | 0.714 |
Rmeas | 0.064 | 0.754 |
Rpim | 0.019 | 0.232 |
Total number of observations | 695835 | |
Number of reflections | 64330 | 2917 |
<I/σ(I)> | 16.2 | |
Completeness [%] | 95.6 | 87.3 |
Redundancy | 10.8 | 9.7 |
CC(1/2) | 0.819 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.2M Sodium acetate trihydrate, 0.1M Tris pH 8.5 30% PEG 4000 |