7YST
Crystal Structure of UDP-glucose 4-epimerase (Rv3634c) in complex with both UDP-glucose and UDP-galactose in chain B from Mycobacterium tuberculosis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E+ SUPERBRIGHT |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2020-03-18 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 55.626, 81.385, 146.363 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.460 - 1.880 |
R-factor | 0.1675 |
Rwork | 0.165 |
R-free | 0.20930 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7ys8 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.920 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.910 |
High resolution limit [Å] | 1.880 | 1.880 |
Rmerge | 0.154 | 1.261 |
Rmeas | 0.160 | 1.317 |
Rpim | 0.043 | 0.374 |
Total number of observations | 765291 | |
Number of reflections | 54985 | 2713 |
<I/σ(I)> | 6.1 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 13.9 | 12.1 |
CC(1/2) | 0.762 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.2M Sodium acetate trihydrate, 0.1M Tris pH 8.5 30% PEG 4000 |