7YSK
Crystal structure of D-Cysteine desulfhydrase from Pectobacterium atrosepticum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2022-01-07 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9785 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.474, 86.540, 68.482 |
| Unit cell angles | 90.00, 90.45, 90.00 |
Refinement procedure
| Resolution | 37.650 - 1.800 |
| R-factor | 0.225 |
| Rwork | 0.223 |
| R-free | 0.26760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4d97 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.914 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.870 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.096 | 0.681 |
| Number of reflections | 55529 | 3320 |
| <I/σ(I)> | 11.9 | 2.2 |
| Completeness [%] | 98.0 | 99.6 |
| Redundancy | 5.3 | 5.7 |
| CC(1/2) | 0.985 | 0.764 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289.15 | 20% (w/v) PEG6000, 100 mM MES/sodium hydroxide pH 6.0, 200 mM ammonium chloride |
