7YSI
Crystal structure of thioredoxin 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 125 |
| Detector technology | PIXEL |
| Collection date | 2022-03-01 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 34.590, 55.010, 78.090 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.418 - 1.202 |
| R-factor | 0.1755 |
| Rwork | 0.175 |
| R-free | 0.18750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3p2a |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.420 | 27.420 |
| High resolution limit [Å] | 1.202 | 1.202 |
| Rmerge | 0.094 | 2.435 |
| Number of reflections | 45597 | 4106 |
| <I/σ(I)> | 15.42 | |
| Completeness [%] | 96.6 | |
| Redundancy | 12.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293.15 | 0.1M HEPES/NaOH pH 7.0, 15% PEG 20000 |
