7YSA
Crystal Structure of UDP-glucose 4-epimerase (Rv3634c) in complex with UDP-glucose in chain A and UDP-galactose in chain B from Mycobacterium tuberculosis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E+ SUPERBRIGHT |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2020-03-06 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 51.222, 76.452, 79.961 |
Unit cell angles | 90.00, 91.97, 90.00 |
Refinement procedure
Resolution | 28.820 - 1.950 |
R-factor | 0.1691 |
Rwork | 0.167 |
R-free | 0.20860 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7ys8 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.984 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | 2.020 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.106 | 0.489 |
Rmeas | 0.118 | 0.573 |
Rpim | 0.052 | 0.290 |
Total number of observations | 224951 | |
Number of reflections | 44546 | 3852 |
<I/σ(I)> | 8.2 | |
Completeness [%] | 98.5 | 85.8 |
Redundancy | 5 | 3.3 |
CC(1/2) | 0.790 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M Sodium acetate trihydrate, 0.1M Tris pH 8.5 30% PEG 4000 |