7YS8

Crystal Structure of UDP-glucose 4-epimerase (Rv3634c) from Mycobacterium tuberculosis

Replaces:  6LTT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2019-11-28
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	50.866, 76.850, 80.124
Unit cell angles	90.00, 91.44, 90.00

Refinement procedure

Resolution	25.230 - 2.020
R-factor	0.1588
Rwork	0.156
R-free	0.20320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4zrm
RMSD bond length	0.003
RMSD bond angle	0.937
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.050
High resolution limit [Å]	2.020	2.020
Rmerge	0.148	0.610
Rmeas	0.164	0.699
Rpim	0.070	0.333
Total number of observations	215119
Number of reflections	40386	1843
<I/σ(I)>	6.7
Completeness [%]	99.5	92.1
Redundancy	5.3	3.9
CC(1/2)		0.735

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	0.2M Sodium acetate trihydrate, 0.1M Tris pH 8.5 30% PEG 4000