7YRT
Crystal structure of PAS like domain of FlrB, the histidine kinase involved in flagellar synthesis of Vibrio cholerae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-05-04 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.972423 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 212.644, 49.556, 146.830 |
Unit cell angles | 90.00, 92.53, 90.00 |
Refinement procedure
Resolution | 48.260 - 2.270 |
R-factor | 0.1883 |
Rwork | 0.185 |
R-free | 0.25070 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Alphafold2 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.062 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.260 | 2.350 |
High resolution limit [Å] | 2.270 | 2.270 |
Rmerge | 0.046 | |
Number of reflections | 69328 | 6819 |
<I/σ(I)> | 9.1 | |
Completeness [%] | 97.7 | |
Redundancy | 2.6 | |
CC(1/2) | 0.997 | 0.526 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 0.8 M Ammonium sulfate, 0.1 M Citric Acid pH 5, 10% Glycerol |