7YRO
Crystal structure of mango fucosyltransferase 13
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL45XU |
Synchrotron site | SPring-8 |
Beamline | BL45XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-05-15 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 86.700, 163.610, 274.900 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.860 - 2.420 |
R-factor | 0.2109 |
Rwork | 0.210 |
R-free | 0.24760 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | predicted model |
RMSD bond length | 0.004 |
RMSD bond angle | 0.697 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.560 |
High resolution limit [Å] | 2.420 | 2.420 |
Number of reflections | 149646 | 24409 |
<I/σ(I)> | 8.55 | 1.14 |
Completeness [%] | 100.0 | |
Redundancy | 9.78 | |
CC(1/2) | 0.995 | 0.544 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.2 M Calcium acetate hydrate and 20% w/v Polyethylene glycol 3,350 |