7YRJ

S-adenosylmethionine sensor upstream of mTORC1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-21
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	64.333, 78.896, 100.096
Unit cell angles	90.00, 96.40, 90.00

Refinement procedure

Resolution	63.930 - 2.350
R-factor	0.2423
Rwork	0.240
R-free	0.29140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	alphafold2
RMSD bond length	0.003
RMSD bond angle	0.572
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHENIX
Refinement software	PHENIX (1.20_4459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	99.470	2.470
High resolution limit [Å]	2.350	2.350
Number of reflections	40916	5694
<I/σ(I)>	7.8
Completeness [%]	98.1
Redundancy	6.1
CC(1/2)	0.997	0.929

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		291.15	Sodium citrate tribasic dihydrate, Polyethylene glycol 3350