7YRJ
S-adenosylmethionine sensor upstream of mTORC1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-11-21 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 64.333, 78.896, 100.096 |
Unit cell angles | 90.00, 96.40, 90.00 |
Refinement procedure
Resolution | 63.930 - 2.350 |
R-factor | 0.2423 |
Rwork | 0.240 |
R-free | 0.29140 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | alphafold2 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.572 |
Data reduction software | autoPROC |
Data scaling software | autoPROC |
Phasing software | PHENIX |
Refinement software | PHENIX (1.20_4459) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 99.470 | 2.470 |
High resolution limit [Å] | 2.350 | 2.350 |
Number of reflections | 40916 | 5694 |
<I/σ(I)> | 7.8 | |
Completeness [%] | 98.1 | |
Redundancy | 6.1 | |
CC(1/2) | 0.997 | 0.929 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 291.15 | Sodium citrate tribasic dihydrate, Polyethylene glycol 3350 |