7YRB
UBR box of human UBR6
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-04-20 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1.00003 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.848, 39.359, 45.248 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.470 - 1.510 |
| R-factor | 0.1689 |
| Rwork | 0.166 |
| R-free | 0.19870 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.061 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.063 | 0.834 |
| Number of reflections | 11429 | 559 |
| <I/σ(I)> | 55.47 | |
| Completeness [%] | 99.8 | 99.6 |
| Redundancy | 12.4 | 11.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 294 | 2.2M Ammonium sulfate, 0.1M HEPES sodium pH 7.5, 1% PEG 400 |
