7YQN

Crystal structure of Ecoli malate synthase G

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	100
Detector technology	CCD
Collection date	2020-08-21
Detector	RAYONIX MX300-HS
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	98.935, 101.246, 170.239
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.450 - 1.600
R-factor	0.1565
Rwork	0.156
R-free	0.17250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1p7t
RMSD bond length	0.006
RMSD bond angle	0.835
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.450	1.657
High resolution limit [Å]	1.600	1.600
Number of reflections	221501	21558
<I/σ(I)>	23.3	2
Completeness [%]	98.5	96.78
Redundancy	4.9
CC(1/2)	0.906	0.690

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	19-22% PEG4000, 0.2M NaCl, 0.1M Tris pH 7.6-8.5