7YQ7

Crystal structure of photosystem II expressing psbA3 gene only

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-05-14
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9
Spacegroup name	P 21 21 21
Unit cell lengths	122.804, 228.456, 286.852
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.990 - 1.900
R-factor	0.1482
Rwork	0.147
R-free	0.17710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3wu2
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	19.990	5.660	2.010
High resolution limit [Å]	1.900	4.020	1.900
Rmerge	0.079	0.032	1.212
Rmeas	0.086	0.035	1.311
Total number of observations	4275973
Number of reflections	628112	43199	100740
<I/σ(I)>	15.81	50.8	1.63
Completeness [%]	99.6	99.5	99.4
Redundancy	6.808	6.556	6.823
CC(1/2)	0.999	0.999	0.735

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	6	285	5% PEG 1450, 20mM NaCl, 10mM CaCl2, 40mM MgSO4, 20mM MES BUFFER pH 6.0, MICRO-BATCH METHOD UNDER OIL, TMPERATURE 285K