7YPV
Crystal structure of OrE-ST-F
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-12-24 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 48.714, 77.826, 108.990 |
| Unit cell angles | 89.94, 90.04, 89.99 |
Refinement procedure
| Resolution | 19.899 - 2.415 |
| Rwork | 0.211 |
| R-free | 0.24540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3pp9 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.414 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.510 |
| High resolution limit [Å] | 2.415 | 2.420 |
| Rmerge | 0.081 | 0.441 |
| Rmeas | 0.099 | 0.545 |
| Number of reflections | 59578 | 5876 |
| <I/σ(I)> | 15.2 | 3.8 |
| Completeness [%] | 97.9 | |
| Redundancy | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1 M MES pH 6.5 0.2 M ammonium sulfate 20% PEG 8000 |
