7YPR

Structural basis of a superoxide dismutase from a tardigrade, Ramazzottius varieornatus strain YOKOZUNA-1.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-27
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9
Spacegroup name	P 32
Unit cell lengths	105.825, 105.825, 76.956
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.866 - 2.101
Rwork	0.180
R-free	0.22810
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.487
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.890	2.160
High resolution limit [Å]	2.100	2.100
Rmerge	0.051	0.225
Rmeas	0.057	0.256
Rpim	0.026	0.121
Number of reflections	56839	4490
<I/σ(I)>	18.4	7
Completeness [%]	99.6	100
Redundancy	4.6	4.4
CC(1/2)	0.999	0.954

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293.15	18% PEG 6000, 0.1 M MES pH 6.5, 0.2 M Potassium chloride, and TCEP addictive