7YPP

Structural basis of a superoxide dismutase from a tardigrade, Ramazzottius varieornatus strain YOKOZUNA-1.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-17
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.28
Spacegroup name	P 1
Unit cell lengths	66.102, 68.049, 68.135
Unit cell angles	94.89, 108.39, 114.40

Refinement procedure

Resolution	45.300 - 2.200
Rwork	0.198
R-free	0.23430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1to4
RMSD bond length	0.008
RMSD bond angle	1.241
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MrBUMP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.370	2.270
High resolution limit [Å]	2.200	2.200
Rmerge	0.100	0.677
Rmeas	0.142	0.957
Rpim	0.100	0.677
Number of reflections	48587	13486
<I/σ(I)>	9.4
Completeness [%]	95.6	93.1
Redundancy	3.6	3.3
CC(1/2)	0.994	0.596

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293.15	26% PEG 8000, 0.1 M MES pH 6.0, 0.2 M calcium acetate, TCEP addictive