7YPH
Open-spiral pentamer of the substrate-free Lon protease with a Y224S mutation
EM specimen
Vitrification | Yes |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Cs (mm) | 2.7 |
Detector | GATAN K3 BIOQUANTUM (6k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 3.68 |
Number of Particle | 4721627 |
Software | cryoSPARC,Coot,EPU,UCSF ChimeraX,PHENIX |
Symmetry | C1 |
CTF correction | PHASE FLIPPING ONLY |