7YPF

Crystal structure of AsfvPCNA in space group of P1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	CCD
Collection date	2020-11-26
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.9793
Spacegroup name	P 1
Unit cell lengths	54.659, 118.873, 118.843
Unit cell angles	60.03, 89.98, 89.96

Refinement procedure

Resolution	29.730 - 2.500
R-factor	0.2094
Rwork	0.208
R-free	0.23250
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	1.019
Data reduction software	iMOSFLM
Data scaling software	HKL-2000
Phasing software	AutoSol
Refinement software	PHENIX ((1.19.1_4122: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.590
High resolution limit [Å]	2.500	5.380	2.500
Rmerge	0.052	0.039	0.406
Rmeas	0.061	0.045	0.496
Rpim	0.032	0.024	0.280
Total number of observations	286920
Number of reflections	87599	8900	8393
<I/σ(I)>	11.2
Completeness [%]	97.5	99	93.3
Redundancy	3.3	3.5	2.8
CC(1/2)		0.995	0.680

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1 M Sodium chloride, 0.1 M BICINE pH 9.0, 20% v/v PEG550