7YPE
Crystal structure of AsfvPCNA in space group of P63
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2020-11-26 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.9793 |
Spacegroup name | P 63 |
Unit cell lengths | 118.660, 118.660, 54.510 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.660 - 2.200 |
R-factor | 0.2098 |
Rwork | 0.209 |
R-free | 0.22690 |
Structure solution method | SAD |
Data reduction software | MOSFLM |
Data scaling software | Aimless (0.5.29) |
Phasing software | AutoSol |
Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 29.660 | 29.660 | 2.270 |
High resolution limit [Å] | 2.200 | 9.070 | 2.200 |
Rmerge | 0.126 | 0.039 | 1.363 |
Rmeas | 0.130 | 0.040 | 1.403 |
Rpim | 0.030 | 0.009 | 0.332 |
Total number of observations | 410355 | 6670 | 34609 |
Number of reflections | 22459 | 341 | 1945 |
<I/σ(I)> | 18.9 | 63.1 | 2.6 |
Completeness [%] | 100.0 | 97.4 | 100 |
Redundancy | 18.3 | 19.6 | 17.8 |
CC(1/2) | 0.999 | 0.999 | 0.758 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1 M Tris pH 8.5 and 20% (w/v) PEG1000 |