7YP6
Crystal structure of elaiophylin glycosyltransferase in complex with UDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-09-29 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 2 2 |
| Unit cell lengths | 66.796, 131.856, 225.173 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 64.120 - 2.600 |
| R-factor | 0.2134 |
| Rwork | 0.212 |
| R-free | 0.24680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7yp3 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.529 |
| Data reduction software | DIALS |
| Data scaling software | SCALA (3.3.16) |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 65.230 | 65.230 | 2.670 |
| High resolution limit [Å] | 2.600 | 11.630 | 2.600 |
| Rmerge | 0.104 | 0.026 | 1.111 |
| Rmeas | 0.120 | 0.030 | 1.280 |
| Rpim | 0.059 | 0.016 | 0.627 |
| Total number of observations | 249252 | 2727 | 18448 |
| Number of reflections | 62124 | 791 | 4553 |
| <I/σ(I)> | 8.4 | 23.8 | 1.3 |
| Completeness [%] | 99.9 | 98.2 | 100 |
| Redundancy | 4 | 3.4 | 4.1 |
| CC(1/2) | 0.996 | 0.996 | 0.524 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.2 | 293 | 21% polyethylene glycol 3350, 0.15 M DL-Malic pH 7.2, 24% propylene glycol |
