7YP5
Crystal structure of elaiophylin glycosyltransferase in complex with TDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-09-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 106.210, 106.210, 215.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 75.210 - 2.330 |
| R-factor | 0.2175 |
| Rwork | 0.215 |
| R-free | 0.25910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7yp3 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.358 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless (0.5.9) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 95.280 | 95.280 | 2.410 |
| High resolution limit [Å] | 2.330 | 9.320 | 2.330 |
| Rmerge | 0.214 | 0.059 | 2.612 |
| Rmeas | 0.219 | 0.060 | 2.674 |
| Rpim | 0.043 | 0.012 | 0.547 |
| Total number of observations | 1240470 | 23719 | 104850 |
| Number of reflections | 50754 | 976 | 4758 |
| <I/σ(I)> | 10.2 | 24 | 1.2 |
| Completeness [%] | 95.1 | 99.8 | 98.3 |
| Redundancy | 24.4 | 24.3 | 22 |
| CC(1/2) | 0.997 | 0.999 | 0.554 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.2 | 293 | 22% polyethylene glycol 3350, 0.15 M DL-Malic pH 7.2, 25% propylene glycol |
