7YP4

Crystal structure of elaiophylin glycosyltransferase in apo-form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2011-04-08
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9791
Spacegroup name	P 21 2 2
Unit cell lengths	66.370, 131.210, 225.290
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	65.690 - 3.100
R-factor	0.2163
Rwork	0.214
R-free	0.26530
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	7yp3
Data reduction software	iMOSFLM
Data scaling software	SCALA (3.3.16)
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0352)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	85.470	85.470	3.240
High resolution limit [Å]	3.100	10.740	3.100
Rmerge	0.140	0.034	0.963
Rmeas	0.161	0.040	1.102
Rpim	0.078	0.019	0.529
Total number of observations	152755	3874	18899
Number of reflections	36576	1017	4387
<I/σ(I)>	7.7	18.7	1.7
Completeness [%]	99.8	99.8	100
Redundancy	4.2	3.8	4.3
CC(1/2)	0.992	0.997	0.534

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.2	293	19% polyethylene glycol 3350, 0.15 M DL-Malic pH 7.2, 18% propylene glycol