7YP3
Crystal structure of elaiophylin glycosyltransferase in complex with elaiophylin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-09-29 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97948 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 228.098, 98.134, 176.506 |
| Unit cell angles | 90.00, 103.26, 90.00 |
Refinement procedure
| Resolution | 76.010 - 2.100 |
| R-factor | 0.1897 |
| Rwork | 0.188 |
| R-free | 0.21690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3d0r |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.351 |
| Data reduction software | DIALS |
| Data scaling software | Aimless (0.5.12) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 76.690 | 76.690 | 2.140 |
| High resolution limit [Å] | 2.100 | 11.500 | 2.100 |
| Rmerge | 0.071 | 0.030 | 0.805 |
| Rmeas | 0.087 | 0.037 | 1.045 |
| Rpim | 0.048 | 0.021 | 0.656 |
| Total number of observations | 659590 | 4299 | 19122 |
| Number of reflections | 217985 | 1414 | 9452 |
| <I/σ(I)> | 8.3 | 24.2 | 1 |
| Completeness [%] | 98.7 | 98.6 | 87 |
| Redundancy | 3 | 3 | 2 |
| CC(1/2) | 0.996 | 0.993 | 0.564 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.8 | 293 | 5% glycerol 3% PEG 3350 1.7M NH4OAC 0.1M Tris pH 7.8 5% Pentaerythritol ethoxylate |
