7YP0

Crystal structure of CtGST

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-28
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	1.0
Spacegroup name	I 21 21 21
Unit cell lengths	81.428, 133.870, 143.099
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.870 - 2.300
R-factor	0.1881
Rwork	0.186
R-free	0.21910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7yoc
RMSD bond length	0.009
RMSD bond angle	1.084
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.340
High resolution limit [Å]	2.300	2.300
Number of reflections	34935	1707
<I/σ(I)>	16
Completeness [%]	1.0
Redundancy	12.1
CC(1/2)	0.999	0.782

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.1 M sodium acetate (pH 4.6), 1.2 M ammonium phosphate monobasic, 0.3 M sodium iodide