7YOR

Recj2 and dCMP complex from Methanocaldococcus jannaschii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-27
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9791
Spacegroup name	P 21 21 2
Unit cell lengths	83.600, 175.170, 66.180
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	75.450 - 2.530
R-factor	0.1922
Rwork	0.188
R-free	0.25340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7yik
RMSD bond length	0.008
RMSD bond angle	0.924
Data reduction software	XDS
Data scaling software	Aimless (0.5.31)
Phasing software	PHENIX
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	175.170	2.600
High resolution limit [Å]	2.530	2.530
Rmerge	0.094	0.662
Rmeas	0.106	0.742
Rpim	0.048	0.330
Total number of observations		11823
Number of reflections	61795	2392
<I/σ(I)>	12	2.6
Completeness [%]	99.8
Redundancy	4.8	4.9
CC(1/2)	0.996	0.758

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	12% PEG 400, 50mM HEPES pH 7.6