7YOQ
Recj2 and CMP complex from Methanocaldococcus jannaschii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-27 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 83.830, 175.630, 66.440 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.907 - 2.265 |
| R-factor | 0.2042 |
| Rwork | 0.202 |
| R-free | 0.25030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7yik |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.908 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.31) |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 175.630 | 175.630 | 2.320 |
| High resolution limit [Å] | 2.260 | 10.130 | 2.260 |
| Rmerge | 0.083 | 0.042 | 0.653 |
| Rmeas | 0.094 | 0.049 | 0.731 |
| Rpim | 0.043 | 0.025 | 0.324 |
| Total number of observations | 1870 | 16673 | |
| Number of reflections | 87358 | 533 | 3382 |
| <I/σ(I)> | 12.1 | 23 | 2.7 |
| Completeness [%] | 99.8 | 86.5 | 100 |
| Redundancy | 4.8 | 3.5 | 4.9 |
| CC(1/2) | 0.996 | 0.994 | 0.799 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 12% PEG 400, 50mM HEPES pH 7.6 |
