7YOM
Crystal structure of tetra mutant (D67E,A68P,L98I,A301S) of O-acetylserine sulfhydrylase from Salmonella typhimurium in complex with high-affinity inhibitory peptide from serine acetyltransferase of Salmonella typhimurium at 2.8 A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2012-05-08 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.54179 |
Spacegroup name | I 41 |
Unit cell lengths | 113.140, 113.140, 43.740 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.831 - 2.800 |
Rwork | 0.216 |
R-free | 0.27230 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1y7l |
RMSD bond length | 0.007 |
RMSD bond angle | 1.515 |
Data reduction software | HKL-2000 |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.831 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.691 | |
Number of reflections | 69653 | 691 |
<I/σ(I)> | 42.47 | |
Completeness [%] | 97.7 | 100 |
Redundancy | 10.1 | |
CC(1/2) | 0.713 | 0.634 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 278 | 0.1M HEPES, 1.3M Sodium citrate |