7YOI
Crystal structure of I88L single mutant of O-acetylserine sulfhydrylase from Haemophilus influenzae in complex with high-affinity inhibitory peptide from serine acetyltransferase of Salmonella typhimurium at 2.14 A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-09-10 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54179 |
| Spacegroup name | I 41 |
| Unit cell lengths | 112.970, 112.970, 44.520 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.454 - 2.140 |
| Rwork | 0.220 |
| R-free | 0.27140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1y7l |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.506 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.454 | 2.217 |
| High resolution limit [Å] | 2.140 | 2.140 |
| Number of reflections | 15711 | 1218 |
| <I/σ(I)> | 73.27 | |
| Completeness [%] | 97.6 | 76.8 |
| Redundancy | 14.6 | |
| CC(1/2) | 0.873 | 0.770 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 278 | 0.1M HEPES, 1.3M Sodium Citrate |
