7YOH
Crystal structure of I88L single mutant of O-acetylserine sulfhydrylase from Haemophilus influenzae in complex with high-affinity inhibitory peptide from serine acetyltransferase of Haemophilus influenzae at 2.5 A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Collection date | 2012-09-11 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.54179 |
Spacegroup name | I 41 |
Unit cell lengths | 112.750, 112.750, 44.460 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.360 - 2.500 |
Rwork | 0.243 |
R-free | 0.29490 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1y7l |
RMSD bond length | 0.006 |
RMSD bond angle | 1.546 |
Data reduction software | HKL-2000 |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.360 | 2.581 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.790 | |
Number of reflections | 9865 | 970 |
<I/σ(I)> | 126.45 | |
Completeness [%] | 99.5 | 99.69 |
Redundancy | 15.5 | |
CC(1/2) | 0.886 | 0.835 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 278 | 0.1M HEPES, 1.3M Sodium citrate |