7YOF
Crystal Structure of Triple mutant (D67E, A68P, I88L) of O-acetyl-L-serine sulfhydrylase from Haemophilus influenzae at 2.3 A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2014-08-27 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.54179 |
Spacegroup name | I 41 |
Unit cell lengths | 112.200, 112.200, 43.740 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.750 - 2.300 |
Rwork | 0.171 |
R-free | 0.22200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1y7l |
RMSD bond length | 0.007 |
RMSD bond angle | 1.481 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.750 | 2.382 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 235300 | 1178 |
<I/σ(I)> | 66.54 | 13.29 |
Completeness [%] | 100.0 | |
Redundancy | 19.1 | |
CC(1/2) | 0.847 | 0.781 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 278 | 0.1M HEPES, 1.3M sodium citrate |