7YOE
Crystal Structure of A68P single mutant of O-acetyl-L-serine sulfhydrylase from Haemophilus influenzae at 1.88 A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-06-24 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54179 |
| Spacegroup name | I 41 |
| Unit cell lengths | 112.281, 112.281, 43.744 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.700 - 1.880 |
| Rwork | 0.201 |
| R-free | 0.22150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1y7l |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.571 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.700 | 1.947 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Number of reflections | 22383 | 2171 |
| <I/σ(I)> | 14.75 | 2.01 |
| Completeness [%] | 99.6 | 97.48 |
| Redundancy | 1.1 | |
| CC(1/2) | 1.000 | 1.000 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 278 | 0.1M HEPES, 1.3M Sodium citrate |
