7YO7

Bifunctional xylosidase/glucosidase LXYL with intermediate substrate xylose, 5 seconds

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-10
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97915
Spacegroup name	C 2 2 21
Unit cell lengths	80.350, 182.570, 242.160
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	73.540 - 1.810
R-factor	0.2137
Rwork	0.212
R-free	0.25030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6kj0
RMSD bond length	0.019
RMSD bond angle	2.145
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	73.540	1.860
High resolution limit [Å]	1.810	1.810
Rmerge	0.136	1.229
Rmeas	0.148	1.325
Rpim	0.056	0.495
Number of reflections	161417	11837
<I/σ(I)>	10.7	1.9
Completeness [%]	99.9	100
Redundancy	13.3	14
CC(1/2)	0.997	0.794

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289.15	15% PEG3350, 0.1 M Tris-HCl pH8.0, 0.2% MgCl2