7YO7
Bifunctional xylosidase/glucosidase LXYL with intermediate substrate xylose, 5 seconds
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-10 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97915 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 80.350, 182.570, 242.160 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 73.540 - 1.810 |
| R-factor | 0.2137 |
| Rwork | 0.212 |
| R-free | 0.25030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6kj0 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.145 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.540 | 1.860 |
| High resolution limit [Å] | 1.810 | 1.810 |
| Rmerge | 0.136 | 1.229 |
| Rmeas | 0.148 | 1.325 |
| Rpim | 0.056 | 0.495 |
| Number of reflections | 161417 | 11837 |
| <I/σ(I)> | 10.7 | 1.9 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 13.3 | 14 |
| CC(1/2) | 0.997 | 0.794 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289.15 | 15% PEG3350, 0.1 M Tris-HCl pH8.0, 0.2% MgCl2 |
