7YO6
Bifunctional xylosidase/glucosidase LXYL with intermediate substrate xylose for 5 sec
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-06-19 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 0.97915 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 79.594, 181.920, 241.960 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 120.980 - 2.540 |
| R-factor | 0.1941 |
| Rwork | 0.191 |
| R-free | 0.26500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7ey1 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.925 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 200.000 | 2.590 |
| High resolution limit [Å] | 2.539 | 2.539 |
| Rmerge | 0.132 | 0.581 |
| Number of reflections | 57972 | 2855 |
| <I/σ(I)> | 11.6 | 2.1 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 5.9 | 6.2 |
| CC(1/2) | 0.976 | 0.937 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289.15 | 0.2M MgCl2, 15% PEG3350, pH 8.0 Tris-HCl |
