7YNY
Crystal structure of baculovirus LEF-3 from Helicoverpa armigera nucleopolyhedrovirus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-07-01 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9789 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 250.059, 117.738, 128.473 |
| Unit cell angles | 90.00, 108.12, 90.00 |
Refinement procedure
| Resolution | 48.520 - 3.510 |
| R-factor | 0.2386 |
| Rwork | 0.236 |
| R-free | 0.28720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | our cryoEM model |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.674 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.520 | 3.640 |
| High resolution limit [Å] | 3.510 | 3.510 |
| Rmerge | 0.069 | 0.737 |
| Number of reflections | 44026 | 4203 |
| <I/σ(I)> | 13.28 | |
| Completeness [%] | 99.2 | |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 1,200 mM sodium phosphate monobasic, 800 mM potassium phosphate dibasic, 100 mM CAPS/NaOH, pH 10.5, 200 mM lithium sulfate |
