7YNV
Crystal structure of photolysed Hen Egg white LYSOZYME introduced with O-(2-nitrobenzyl)-L-tyrosine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL32XU |
Synchrotron site | SPring-8 |
Beamline | BL32XU |
Temperature [K] | 93 |
Detector technology | PIXEL |
Collection date | 2020-12-11 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 1 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 78.280, 78.280, 37.370 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.140 - 1.390 |
R-factor | 0.1757 |
Rwork | 0.174 |
R-free | 0.19220 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ym8 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 39.140 | 39.140 | 1.470 |
High resolution limit [Å] | 1.390 | 4.170 | 1.390 |
Rmerge | 0.121 | 0.109 | 0.373 |
Rmeas | 0.124 | 0.111 | 0.384 |
Total number of observations | 526644 | ||
Number of reflections | 23873 | 1009 | 3508 |
<I/σ(I)> | 23.06 | 38.8 | 7.01 |
Completeness [%] | 99.5 | 99.7 | 96.8 |
Redundancy | 22.06 | 22.176 | 17.839 |
CC(1/2) | 0.998 | 0.997 | 0.970 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 3.6 | 293 | 28% NaCl, 8% PEG6000, 100 mM Na-acetate (pH3.6) |