7YN3

Crystal structure of CcbD complex with CcbZ carrier protein domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-17
Detector	DECTRIS EIGER X 4M
Wavelength(s)	1.07
Spacegroup name	P 21 21 21
Unit cell lengths	89.572, 90.104, 124.411
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.790 - 2.100
R-factor	0.177
Rwork	0.175
R-free	0.22640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7yn1
RMSD bond length	0.007
RMSD bond angle	0.855
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.790	2.160
High resolution limit [Å]	2.100	2.100
Rmerge	0.098	0.677
Number of reflections	59483	4565
<I/σ(I)>	14.7	2.9
Completeness [%]	100.0	100
Redundancy	7.6	7.9
CC(1/2)	0.998	0.848

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.1 M MES (pH 6.7), 0.16 M magnesium chloride, 0.2 M sodium thiocyanate, 8% w/v PEG 8000