7YN1
Crystal structure of selenomethionine labeled CcbD
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-1A |
Synchrotron site | Photon Factory |
Beamline | BL-1A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-08-12 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 0.9788 |
Spacegroup name | P 1 |
Unit cell lengths | 52.683, 90.932, 98.563 |
Unit cell angles | 90.27, 103.67, 90.19 |
Refinement procedure
Resolution | 26.130 - 2.500 |
R-factor | 0.2043 |
Rwork | 0.203 |
R-free | 0.24030 |
Structure solution method | SAD |
RMSD bond length | 0.002 |
RMSD bond angle | 0.523 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | CRANK2 |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.880 | 2.560 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.093 | 0.580 |
Number of reflections | 60701 | 4419 |
<I/σ(I)> | 18.9 | 4.5 |
Completeness [%] | 98.8 | 98.4 |
Redundancy | 11.8 | 11.9 |
CC(1/2) | 0.999 | 0.935 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.02 M Tris-HCl (pH 6.7), 0.16 M magnesium chloride, 10% w/v PEG 8000. |