7YMO

Crystal structure of the recombination mediator protein RecO from Campylobacter jejuni

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2020-09-26
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	39.147, 61.711, 97.204
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.690 - 1.800
R-factor	0.1802
Rwork	0.179
R-free	0.20800
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	0.775
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AutoSol
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.830
High resolution limit [Å]	1.800	1.800
Number of reflections	22418	1110
<I/σ(I)>	33.1	3.9
Completeness [%]	99.6	99.7
Redundancy	5.7	5.6
CC(1/2)	0.995	0.838

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	PEG 3350, sodium formate