7YLT
Structure of a bacteria protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-10-06 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 |
Unit cell lengths | 36.775, 75.745, 119.706 |
Unit cell angles | 85.60, 89.16, 89.37 |
Refinement procedure
Resolution | 48.670 - 2.300 |
R-factor | 0.1883 |
Rwork | 0.186 |
R-free | 0.22750 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Alphafold2 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.641 |
Data reduction software | autoPROC |
Data scaling software | autoPROC |
Phasing software | PHENIX (1.20_4459) |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.670 | 2.382 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 52101 | 4716 |
<I/σ(I)> | 9.78 | |
Completeness [%] | 91.0 | |
Redundancy | 3.6 | |
CC(1/2) | 0.998 | 0.968 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIQUID DIFFUSION | 291.15 | Polyethylene glycol 3350, Tris, Cobalt(II) chloride hexahydrate |