7YLB
Two monobodies recognizing the conserved epitopes of SARS-CoV-2 N antigen applicable to the broad COVID-19 diagnosis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-12-05 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 143.081, 48.458, 143.247 |
Unit cell angles | 90.00, 105.16, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 138.261 | 2.450 |
High resolution limit [Å] | 2.410 | 2.410 |
Rmerge | 0.186 | 0.945 |
Number of reflections | 74632 | 3673 |
<I/σ(I)> | 7.6 | |
Completeness [%] | 99.8 | |
Redundancy | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 293.15 | 0.2M K2SO4, 18% PEG 3350 |