7YL6
Cell surface protein YwfG protein complexed with alpha-1,2-mannobiose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 95 |
Detector technology | PIXEL |
Collection date | 2020-12-11 |
Detector | DECTRIS PILATUS3 S 2M |
Wavelength(s) | 1.0000 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 43.454, 276.634, 321.750 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.511 - 2.950 |
Rwork | 0.211 |
R-free | 0.26840 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 7yl4 |
RMSD bond length | 0.014 |
RMSD bond angle | 2.033 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.060 | 3.110 |
High resolution limit [Å] | 2.950 | 2.950 |
Rmerge | 0.203 | 1.187 |
Rmeas | 0.211 | 1.232 |
Rpim | 0.058 | 0.329 |
Number of reflections | 42029 | 6070 |
<I/σ(I)> | 14 | 2.5 |
Completeness [%] | 99.9 | |
Redundancy | 13 | |
CC(1/2) | 0.996 | 0.774 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M sodium acetate (pH 5.0-5.6), 1.3 M Li2SO4, 2% (w/v) polyethylene glycol 3350 |