7YKN

Crystal structure of (6-4) photolyase from Vibrio cholerae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU PhotonJet-R
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-10
Detector	RIGAKU HyPix-3000
Wavelength(s)	1.54
Spacegroup name	P 64 2 2
Unit cell lengths	200.751, 200.751, 77.023
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.620 - 2.500
R-factor	0.2274
Rwork	0.225
R-free	0.28320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4dja
Data reduction software	CrysalisPro
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.620	2.580
High resolution limit [Å]	2.500	2.500
Rmerge	1.382	3.765
Rmeas	1.394	3.796
Rpim	0.178	0.485
Number of reflections	32043	3130
<I/σ(I)>	5.61	1.7
Completeness [%]	99.6	99.97
Redundancy	60.4
CC(1/2)	0.955	0.546

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH		277	5%(v/v) PEG400, 2 M ammonium citrate/citric acid pH 7.5, 1 M sodium sulfate decahydrate