7YKM

Structure of DciA DUF721 domain from Deinococcus radiodurans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2021-11-20
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.9762
Spacegroup name	C 1 2 1
Unit cell lengths	92.593, 37.438, 86.285
Unit cell angles	90.00, 164.34, 90.00

Refinement procedure

Resolution	24.767 - 1.500
R-factor	0.193799119145
Rwork	0.193
R-free	0.21523
RMSD bond length	0.007
RMSD bond angle	1.001
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.340	1.530
High resolution limit [Å]	1.500	1.500
Rmerge	0.087	0.441
Number of reflections	12543	541
<I/σ(I)>	8.9
Completeness [%]	96.7
Redundancy	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	289	2.0 M Ammonium nitrate, 0.1 M Sodium acetate trihydrate pH 5.0, 200 mM NaCl