7YKF
Crystal structure of MAGI2 PDZ0-GK/pEphexin4 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-06-07 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97737 |
Spacegroup name | P 1 |
Unit cell lengths | 40.209, 55.616, 61.070 |
Unit cell angles | 65.31, 76.62, 71.56 |
Refinement procedure
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 2.340 |
High resolution limit [Å] | 2.280 | 6.230 | 2.280 |
Rmerge | 0.068 | 0.039 | 0.317 |
Rmeas | 0.081 | 0.046 | 0.380 |
Rpim | 0.043 | 0.025 | 0.207 |
Number of reflections | 19920 | 1004 | 1005 |
<I/σ(I)> | 6.3 | ||
Completeness [%] | 97.5 | 98.4 | 95.9 |
Redundancy | 3.3 | 3.4 | 3.1 |
CC(1/2) | 0.996 | 0.954 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.2 M magnesium chloride hexahydrate, 0.1 M Tris (pH 8.5), 25% w/v PEG 3350 |