7YIL
The structure of GINS from Methanocaldococcus jannaschii
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-07-24 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.979 |
Spacegroup name | P 64 2 2 |
Unit cell lengths | 67.410, 67.410, 236.560 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 32.670 - 2.170 |
R-factor | 0.2183 |
Rwork | 0.217 |
R-free | 0.24500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5GHS and 2E9X |
Data reduction software | xia2 |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 33.790 | 33.790 | 2.230 |
High resolution limit [Å] | 2.170 | 9.710 | 2.170 |
Rmerge | 0.078 | 0.048 | 1.576 |
Rmeas | 0.079 | 0.049 | 1.603 |
Rpim | 0.013 | 0.009 | 0.287 |
Total number of observations | 638608 | 6685 | 38718 |
Number of reflections | 17808 | 265 | 1269 |
<I/σ(I)> | 26.7 | 53.9 | 2.5 |
Completeness [%] | 99.7 | 97.6 | 99.7 |
Redundancy | 35.9 | 25.2 | 30.5 |
CC(1/2) | 0.999 | 0.999 | 0.894 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20.1% PEG 1500, 5.36% MPD, 0.1M Tris-Base pH8.5 |