7YIB

Crystal structure of wild-type Cap4 SAVED domain-containing receptor from Enterobacter cloacae

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	100
Detector technology	CCD
Collection date	2021-03-09
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979, 0.979, 0.954
Spacegroup name	P 21 21 2
Unit cell lengths	150.958, 70.947, 56.468
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.440 - 2.180
R-factor	0.1673
Rwork	0.166
R-free	0.19980
Structure solution method	MAD
RMSD bond length	0.002
RMSD bond angle	0.566
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.17.1_3660)
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.260
High resolution limit [Å]	2.180	2.180
Rmerge	0.057	0.351
Number of reflections	60956	3126
<I/σ(I)>	43.13	5.95
Completeness [%]	99.6	97.3
Redundancy	11.4	10.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.1 M MES pH 6.0, 0.2 M sodium chloride, 20% PEG 3350