7YH5
MazG(Mycobacterium tuberculosis)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 291 |
Detector technology | CCD |
Collection date | 2019-11-01 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9792 |
Spacegroup name | P 4 21 2 |
Unit cell lengths | 109.310, 109.310, 242.910 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.860 - 2.700 |
R-factor | 0.2599 |
Rwork | 0.258 |
R-free | 0.29560 |
Structure solution method | AB INITIO PHASING |
RMSD bond length | 0.006 |
RMSD bond angle | 0.939 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.860 | 2.796 |
High resolution limit [Å] | 2.700 | 2.700 |
Number of reflections | 41350 | 41350 |
<I/σ(I)> | 17.9 | |
Completeness [%] | 99.8 | |
Redundancy | 2.8 | |
CC(1/2) | 1.000 | 1.000 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1 M HEPES pH 7.5, 10% W/V PEG8000, 8% Ethylene glycol |